近日，Nature發(fā)表了中科院微生物所關(guān)于新冠中和抗體的重大研究成果，他們從康復(fù)患者篩選出了兩株全人源單克隆抗體CB6和CA1， 其中CB6克隆在非人靈長類動物試驗?zāi)Ｐ椭心軌蛴行е委熀皖A(yù)防新冠病毒感染。目前國家藥品監(jiān)督管理局已經(jīng)正式批準CB6（JS016）的臨床試驗申請。CR3022單抗克隆株是一株從非典肺炎康復(fù)患者體內(nèi)分離出的全人源化單克隆抗體。這株抗體雖然可以和新冠病毒交叉反應(yīng)，但是不具備中和和抑制病毒感染的功能，是一株作為廣泛研究的參照抗體。
Figure 1. CB6 can block SARS-CoV2-RBD binding to hACE2 in FACS-based assay
(圖片來源：Nature)
目前檢測新冠病毒的主要方法是核酸檢測和免疫學檢測，采取核酸檢測為主，免疫學檢測為輔的策略。其中免疫學檢測分為抗原檢測和抗體檢測兩種，對于免疫學檢測來說，優(yōu)質(zhì)的抗原抗體對于檢測的靈敏度和特異性至關(guān)重要。締碼生物應(yīng)用HEK293人源細胞表達平臺制備的重組新冠病毒S蛋白更接近于天然翻譯后修飾，并且我們也對表達純化的S蛋白測試進行了ACE2受體蛋白結(jié)合功能驗證和多種已發(fā)表新冠病毒中和性單克隆抗體結(jié)合功能驗證（包括最新的CB6和CR3022單抗克?。?。
CB6單克隆抗體可以有效的抑制新冠病毒S-RBD蛋白結(jié)合ACE2受體蛋白
Figure 2. ELISA plate coated with recombinant SARA-CoV-2 S protein RBD was first pre-blocked with recombinant CB6 antibody or recombinant CR3022 antibody, and then 100 ng/ml Human ACE2, mFc tagged protein (PME100072) was added to check the competitive inhibition of recombinant CB6 antibody and recombinant CR3022 antibody. This data indicated that recombinant CB6 antibody can efficiently block the binding between SARS-CoV-2 S protein RBD domain with Human ACE2 protein.
好禮贈送
為了方便客戶進行蛋白功能驗證和應(yīng)用CB6和CR3022作為參照抗體進行研發(fā)，我們所有銷售的新冠蛋白產(chǎn)品都會附送重組表達的CB6和CR3022單克隆抗體。
產(chǎn)品推薦
SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His Tag
Catalog Number: PME100460
Expression Host: HEK293
Tag: C-Mouse Fc and 6×His tag
Purity: Greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Figure 3. SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 4. ELISA plate pre-coated by 2 μg/ml (100 μl/well) SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His tagged protein (PME100460) can bind Anti-SARS-CoV-2 S protein RBD Neutralizing Antibody (A neutralizing monoclonal antibody clone currently under clinical investigation from collaboration company) in a linear range of 0.24-9.141 ng/ml.
Human ACE2 Protein, mFc Tag
Catalog Number: PME100072
Expression Host: HEK293
Tag: C-Mouse Fc tag
Purity: Greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Figure5.Human ACE2 Protein, mFc Tag on SDS-PAGE under reducing condition.
SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein, His Tag
Catalog Number: PME100459
Expression Host: E.coli
Tag: C-His tag
Purity: Greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Figure 6.?SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein, His Tag on SDS-PAGE under reducing condition.除了重組蛋白產(chǎn)品的開發(fā)，我們的締碼重組單克隆抗體制備平臺也正在應(yīng)用于新冠病毒相關(guān)重組單克隆抗體開發(fā)。與傳統(tǒng)的雜交瘤制備平臺相比，我們擁有更高的篩選效率，從單只免疫動物中我們能夠篩選到上千甚至上萬個陽性B細胞克隆，這些陽性克隆能夠幫助我們篩選擁有更多表位和適用于更多免疫學應(yīng)用的抗體。我們的新冠抗體開發(fā)正在進行中，歡迎廣大客戶關(guān)注我們，來電咨詢更多相關(guān)信息。
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